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citrate buffer  (MedChemExpress)


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    Structured Review

    MedChemExpress citrate buffer
    Citrate Buffer, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sodium+citrate+buffer/Sodium+Citrate+Buffer%2C+0%2E1M%2C+pH+4%2E0/pm41692190-105-16-19
    Average 94 stars, based on 8 article reviews
    citrate buffer - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Counting:

    Article Title: Dual-Targeting Mn@CeO 2 Nanozyme-Modified Probiotic Hydrogel Microspheres Reshape Gut Homeostasis in Inflammatory Bowel Disease
    Article Snippet: .. Prior to plate counting or optical density measurement, MnCe@LR/AMs were pretreated with 100 mM sodium citrate buffer (MedChemExpress) for bacterial release. ..

    Injection:

    Article Title: Exosomal miR-2467-3p derived from human umbilical cord mesenchymal stem cells promotes diabetic wound healing by regulating autophagy via targeting CYP1A1.
    Article Snippet: Objective: This study aimed to investigate the molecular mechanisms by which human umbilical cord-derived mesenchymal stem cell exosomes (hUC-MSC-Exo) promote diabetic wound healing by focusing on the delivery of specific microRNAs (miRNAs) that regulate downstream target genes and subsequently activate the

    Article Title: Formononetin in Jiawei Qihuangyin inhibits podocyte epithelial-mesenchymal transition and ameliorates diabetic nephropathy via SIRT1/NF-κB axis.
    Article Snippet: .. To induce DN, 1 % streptozotocin (STZ) (Macklin, China) was injected intraperitoneally into rats at a dose of 45 mg/kg (dissolved in 0.1 M citrate buffer, pH 4.5), and the NC group was treated with the same amount of sodium citrate buffer (MCE, China). ..

    Immunofluorescence:

    Article Title: Asymmetrical paravertebral muscles fibrosis causes progression of adolescent idiopathic scoliosis via myostatin signalling in fibro-adipogenic progenitors
    Article Snippet: For haematoxylin and eosin (H&E) staining, sections were deparaffinized, rehydrated, and stained with haematoxylin (#C0107, Beyotime) for 5 min and eosin (#C0109, Beyotime) for 2 min. Fibrosis was quantified using Sirius Red staining (#Y026186, Beyotime) in three random fields per section using ImageJ (NIH, Maryland, USA) by independent double-blinded investigators. .. For immunofluorescence, sections underwent antigen retrieval in sodium citrate buffer (#HY-B1610N, MCE) at 37 °C for 60 min, peroxidase quenching, blocking with 5 % bovine serum albumin (BSA, #ST2254, Beyotime). .. Sections were incubated overnight at 4 °C with antibodies: Mouse anti-PDGFRA (1:200, #14-1401-82, Thermo Fisher Scientific), Rabbit anti-laminin (1:200, #ab11575, Abcam), Mouse anti-collagen 1 (1:200, #67288-1-Ig, ProteinTech), Rabbit anti-myostatin (1:200, #19142-1-AP, ProteinTech), and Rabbit anti-p-SMAD3 (1:250, #44-246G, Thermo Fisher Scientific).

    Article Title: Asymmetrical paravertebral muscles fibrosis causes progression of adolescent idiopathic scoliosis via myostatin signalling in fibro-adipogenic progenitors
    Article Snippet: For haematoxylin and eosin (H&E) staining, sections were deparaffinized, rehydrated, and stained with haematoxylin (#C0107, Beyotime) for 5 min and eosin (#C0109, Beyotime) for 2 min. Fibrosis was quantified using Sirius Red staining (#Y026186, Beyotime) in three random fields per section using ImageJ (NIH, Maryland, USA) by independent double-blinded investigators. .. For immunofluorescence, sections underwent antigen retrieval in sodium citrate buffer (#HY-B1610N, MCE) at 37 ◦C for 60 min, peroxidase quenching, blocking with 5 % bovine serum albumin (BSA, #ST2254, Beyotime). .. Sections were incubated overnight at 4 ◦C with antibodies: Mouse anti-PDGFRA (1:200, #14-1401-82, Thermo Fisher Scientific), Rabbit anti-laminin (1:200, #ab11575, Abcam), Mouse anti-collagen 1 (1:200, #67288-1-Ig, ProteinTech), Rabbit anti-myostatin (1:200, #19142-1-AP, ProteinTech), and Rabbit anti-p-SMAD3 (1:250, #44- 246G, Thermo Fisher Scientific).

    Blocking Assay:

    Article Title: Asymmetrical paravertebral muscles fibrosis causes progression of adolescent idiopathic scoliosis via myostatin signalling in fibro-adipogenic progenitors
    Article Snippet: For haematoxylin and eosin (H&E) staining, sections were deparaffinized, rehydrated, and stained with haematoxylin (#C0107, Beyotime) for 5 min and eosin (#C0109, Beyotime) for 2 min. Fibrosis was quantified using Sirius Red staining (#Y026186, Beyotime) in three random fields per section using ImageJ (NIH, Maryland, USA) by independent double-blinded investigators. .. For immunofluorescence, sections underwent antigen retrieval in sodium citrate buffer (#HY-B1610N, MCE) at 37 °C for 60 min, peroxidase quenching, blocking with 5 % bovine serum albumin (BSA, #ST2254, Beyotime). .. Sections were incubated overnight at 4 °C with antibodies: Mouse anti-PDGFRA (1:200, #14-1401-82, Thermo Fisher Scientific), Rabbit anti-laminin (1:200, #ab11575, Abcam), Mouse anti-collagen 1 (1:200, #67288-1-Ig, ProteinTech), Rabbit anti-myostatin (1:200, #19142-1-AP, ProteinTech), and Rabbit anti-p-SMAD3 (1:250, #44-246G, Thermo Fisher Scientific).

    Article Title: Asymmetrical paravertebral muscles fibrosis causes progression of adolescent idiopathic scoliosis via myostatin signalling in fibro-adipogenic progenitors
    Article Snippet: For haematoxylin and eosin (H&E) staining, sections were deparaffinized, rehydrated, and stained with haematoxylin (#C0107, Beyotime) for 5 min and eosin (#C0109, Beyotime) for 2 min. Fibrosis was quantified using Sirius Red staining (#Y026186, Beyotime) in three random fields per section using ImageJ (NIH, Maryland, USA) by independent double-blinded investigators. .. For immunofluorescence, sections underwent antigen retrieval in sodium citrate buffer (#HY-B1610N, MCE) at 37 ◦C for 60 min, peroxidase quenching, blocking with 5 % bovine serum albumin (BSA, #ST2254, Beyotime). .. Sections were incubated overnight at 4 ◦C with antibodies: Mouse anti-PDGFRA (1:200, #14-1401-82, Thermo Fisher Scientific), Rabbit anti-laminin (1:200, #ab11575, Abcam), Mouse anti-collagen 1 (1:200, #67288-1-Ig, ProteinTech), Rabbit anti-myostatin (1:200, #19142-1-AP, ProteinTech), and Rabbit anti-p-SMAD3 (1:250, #44- 246G, Thermo Fisher Scientific).

    Incubation:

    Article Title: Dual-Targeting Mn@CeO 2 Nanozyme-Modified Probiotic Hydrogel Microspheres Reshape Gut Homeostasis in Inflammatory Bowel Disease.
    Article Snippet: .. Prior to plate counting or optical density measurement, MnCe@ LR/AMs were pretreated with 100 mM sodium citrate buffer (MedChemExpress) for bacterial release.67 Following 30 min incubation at room temperature, the samples were washed twice with PBS (pH 7.4) via centrifugation (3,000 × g, 5 min). .. Equal amounts of native LR, MnCe@LR, and MnCe@LR/AMs were individually resuspended in simulated gastric fluid (SGF, pH = 1.3, purchased from Shanghai Yuanye Bio-Technology Co., Ltd.), simulated intestinal fluid (SIF, pH = 6.8, containing trypsin and phosphates, purchased from Shanghai Yuanye Bio-Technology Co., Ltd.), simulated colon fluid (SCF, pH = 7.4, purchased from Shanghai Yuanye Bio-Technology Co., Ltd.) and H2O2 (5 mM), and then incubated at 37 °C with gentle shaking.

    Centrifugation:

    Article Title: Dual-Targeting Mn@CeO 2 Nanozyme-Modified Probiotic Hydrogel Microspheres Reshape Gut Homeostasis in Inflammatory Bowel Disease.
    Article Snippet: .. Prior to plate counting or optical density measurement, MnCe@ LR/AMs were pretreated with 100 mM sodium citrate buffer (MedChemExpress) for bacterial release.67 Following 30 min incubation at room temperature, the samples were washed twice with PBS (pH 7.4) via centrifugation (3,000 × g, 5 min). .. Equal amounts of native LR, MnCe@LR, and MnCe@LR/AMs were individually resuspended in simulated gastric fluid (SGF, pH = 1.3, purchased from Shanghai Yuanye Bio-Technology Co., Ltd.), simulated intestinal fluid (SIF, pH = 6.8, containing trypsin and phosphates, purchased from Shanghai Yuanye Bio-Technology Co., Ltd.), simulated colon fluid (SCF, pH = 7.4, purchased from Shanghai Yuanye Bio-Technology Co., Ltd.) and H2O2 (5 mM), and then incubated at 37 °C with gentle shaking.



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